Comparison of three different methods in [⁶⁸ Ga]Ga-PSMA11 radiolabeling

Area of Science:

• Nuclear Medicine
• Radiopharmaceutical Chemistry
• Oncology

Background:

• Prostate-specific membrane antigen (PSMA) targeted positron emission tomography (PET) imaging is crucial for prostate cancer management.
• Gallium-68 (Ga-68) labeled PSMA tracers like Ga-68 PSMA11 are widely used for PET imaging.
• Optimizing Ga-68 PSMA11 synthesis is essential for clinical accessibility and research.

Purpose of the Study:

• To evaluate and compare quality control parameters of Ga-68 PSMA11 synthesized via three different methods.
• To assess radiochemical yield, purity, and in vitro stability for each synthesis algorithm.
• To identify the most practical and cost-effective labeling method for clinical application.

Main Methods:

• Comparison of three synthesis algorithms for Ga-68 PSMA11 production using a Ga-68/Ga-68 generator.
• Evaluation of an automated synthesis module, an ANMI kit, and direct cold labeling with sodium bicarbonate buffer.
• Assessment of radiochemical yield, radiochemical purity, and in vitro stability for all methods.

Main Results:

• The automated synthesis module and ANMI kit methods met all quality control expectations for clinical use.
• The direct cold labeling with sodium bicarbonate buffer yielded comparable results to the ANMI kit.
• All three methods demonstrated successful Ga-68 complexation and met quality control criteria.

Conclusions:

• Ga-68 PSMA11 can be successfully synthesized using automated modules, commercial kits, or direct labeling with sodium bicarbonate buffer.
• Direct labeling with sodium bicarbonate buffer is identified as a preferred method due to its low cost, practicality, and ease of use.
• The findings support the clinical applicability of Ga-68 PSMA11 synthesized through various accessible methods.