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A quantitative cholinergic and catecholaminergic 3D Atlas of the developing mouse brain
B Riffault1, R Cloarec1, H Rabiei2
1Neurochlore, Campus scientifique de Luminy, Marseille, France.
Neuroimage
|July 23, 2022
Summary
This study presents the first 3D brain atlases for cholinergic and catecholaminergic systems in mice, revealing key developmental changes and providing a tool for future research on neurological disorders.
Area of Science:
- Neuroscience
- Developmental Biology
- Neuroanatomy
Background:
- Brain development involves complex organizational changes requiring advanced visualization techniques.
- Understanding the development of specific neuronal systems is crucial for identifying potential malformations.
Purpose of the Study:
- To create the first comprehensive 3D atlases of the main cholinergic (CH) and catecholaminergic (CA) systems in the developing mouse brain.
- To quantitatively analyze dynamic changes in these neuronal systems from embryonic day 12 (E12) to post-natal day 8 (P8).
Main Methods:
- Utilized tissue clearing, immunohistochemistry, and 3D lightsheet microscopy.
- Employed a multiresolution registration technique for accurate data analysis.
- Developed voxel-wise analysis for detailed examination of brain structure modifications.
Main Results:
- Established 3D atlases for CH and CA systems in the developing mouse brain.
- Observed a logarithmic increase in CH and tyrosine hydroxylase positive neurons from E18 to P8 in several brain structures.
- Identified abrupt developmental trajectory changes in brain structures between E18 and P0.
Conclusions:
- The created 3D atlases serve as a valuable anatomical reference for developmental studies.
- These atlases facilitate quantitative determination of CH or CA neuron fate in utero.
- The resource will aid in studying developmental malformations linked to neurological and psychiatric disorders.
Keywords:
3D quantitative atlasBirthBrain structuresCatecholaminergicCholinergicNeuronal countsTissue clearingin utero
