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Updated: Sep 3, 2025
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Published on: May 27, 2014
Analysis of metabolites in green tea during the roasting process using non-targeted metabolomics
Fei Liu1,2, Zheng Tu3, Lin Chen4
1Tea Research Institute of Sichuan Academy of Agricultural Science, Chengdu, China.
Background:
Roasting plays an important role in the formation of flavor of roasted green tea; however, the changes in chemicals during this process have not been systematically studied until now. To reveal the dynamic changes in chemicals in green tea during roasting, non-targeted metabolomics, coupled with chemometrics, was employed.
Results:
A total of 101 non-volatile metabolites were identified in tea samples, and 29 metabolites were identified as characteristic metabolites of roasting. A significant increase in catechins and their derivatives, organic acids, and flavonoid glycosides was observed, while the content of some amino acids and their derivatives decreased over 50% during roasting. The content of theanine glucoside increased dramatically (by 21.23-fold at the roasting stage), and Maillard-derived compounds also increased to varying degrees.
Conclusion:
Glycosylation, oxidative polymerization, and pyrolysis were important reactions responsible for the formation and transformation of flavor compounds in roasted green tea during roasting. © 2022 Society of Chemical Industry.
