Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

103.1K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
103.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasma GDF15 affects long-term dementia risk and alters neuroimmune signaling.

Science advances·2026
Same author

A new AI assisted approach aligns data standards and accelerates interoperability in biomedical research.

NPJ digital medicine·2026
Same author

Contingency of Plasma Dementia Biomarkers on Cognitive Profiles for Prognosis of Incident Dementia: The ARIC Study.

Neurology·2026
Same author

Support from start to finish-a collaborative primary medical program in rural Victoria, Australia.

Frontiers in medicine·2025
Same author

Plasma GDF15 affects long-term dementia risk and alters neuro-immune signaling.

medRxiv : the preprint server for health sciences·2025
Same author

Association of Body Mass Index in Late Life, and Change from Midlife to Late Life, With Incident Dementia in the ARIC Study Participants.

Neurology·2025

Related Experiment Video

Updated: Sep 3, 2025

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K

Synchronizing spermatogenesis in the mouse.

Michael Griswold1, Cathryn Hogarth2

  • 1School of Molecular Biosciences, Washington State University, Pullman, WA, USA.

Biology of Reproduction
|July 24, 2022
PubMed
Summary

This study synchronizes spermatogenesis using retinoic acid (RA) inhibition and replacement. This method enriches specific germ cell types, simplifying the study of male reproductive cell development.

Keywords:
germ cellsretinoic acidspermatogenesissynchronization

More Related Videos

Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.7K
Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

910

Related Experiment Videos

Last Updated: Sep 3, 2025

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
09:40

A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model

Published on: February 6, 2018

15.2K
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.7K
Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

910

Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Cell Biology

Background:

  • Spermatogenesis, the process of sperm formation, is complex and normally results in a mixture of germ cell types in the adult testis.
  • This heterogeneity makes it challenging to isolate specific cell types or study distinct stages of the seminiferous epithelium cycle.
  • Retinoic acid (RA) is crucial for germ cell differentiation, initiating the process ~35 days prior to mature sperm formation.

Purpose of the Study:

  • To develop a method for synchronizing spermatogenesis to facilitate the study of germ cell development.
  • To enrich specific germ cell types or stages within the seminiferous epithelium.

Main Methods:

  • Utilized WIN 18,446, an inhibitor of retinoic acid (RA) synthesis, to block endogenous RA signaling.
  • Administered exogenous RA to reinitiate and synchronize the differentiation process.
  • Analyzed the resulting germ cell populations during the first wave and in the adult testis.

Main Results:

  • The combined inhibition and RA re-administration successfully synchronized spermatogenesis.
  • This synchronization resulted in the prevalence of only one to four germ cell types during the first wave of spermatogenesis.
  • In adult testes, specific, predictable stages of the seminiferous epithelium cycle were enriched throughout the tissue.

Conclusions:

  • The described method effectively synchronizes spermatogenesis by manipulating retinoic acid levels.
  • This synchronization strategy enriches targeted germ cell types and stages, offering a valuable tool for reproductive biology research.
  • Facilitates the isolation and functional study of specific germ cell populations and seminiferous epithelium stages.