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GAF domain is essential for nitrate-dependent AtNLP7 function.

Jie Wu1, Ying Song2, Zi-Sheng Zhang2

  • 1Division of Life Sciences and Medicine, Division of Molecular & Cell Biophysics, Hefei National Science Center for Physical Sciences at the Microscale, MOE Key Laboratory for Membraneless Organelles and Cellular Dynamics, University of Science and Technology of China, The Innovation Academy of Seed Design, Chinese Academy of Sciences, Hefei, 230027, Anhui Province, China. wujie199104@163.com.

BMC Plant Biology
|July 24, 2022
PubMed
Summary

The GAF domain of the AtNLP7 protein is crucial for plants to sense nitrate deficiency. This domain controls AtNLP7

Keywords:
AtNLP7GAF domainNitrate deficiency signalingNuclear localizationROS

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Area of Science:

  • Plant molecular biology
  • Nutrient signaling

Background:

  • Nitrate is vital for plant growth and acts as a signaling molecule.
  • Understanding nitrate deficiency perception is key to plant science.
  • The precise mechanisms of nitrate signaling remain unclear.

Purpose of the Study:

  • To investigate the role of the N-terminal GAF domain of the AtNLP7 protein in nitrate signaling.
  • To elucidate the molecular mechanisms of nitrate deficiency perception in plants.

Main Methods:

  • Investigated the transport of AtNLP7 protein in response to nitrate deficiency.
  • Utilized AtNLP7 deletion mutants (AtNLP7ΔGAF) to study protein localization and function.
  • Assessed gene activation, DNA binding affinity, and reactive oxygen species (ROS) accumulation.

Main Results:

  • AtNLP7 protein transport from nucleus to cytoplasm depends on the GAF domain.
  • AtNLP7ΔGAF mutant protein remains in the nucleus, impairing nitrate-induced gene activation.
  • The GAF domain is essential for binding to nitrate-responsive cis-elements (NREs) and regulating ROS levels.

Conclusions:

  • The GAF domain of AtNLP7 is critical for sensing nitrate deficiency signals.
  • This domain mediates nitrate-triggered ROS signaling pathways.
  • AtNLP7's GAF domain is essential for proper plant growth under varying nitrate availability.