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Related Experiment Video

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Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
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851

Carotenoid extraction, detection, and analysis in citrus.

Kaijie Zhu1, Hongyan Chen1, Yingzi Zhang1

  • 1Key Laboratory of Horticultural Plant Biology of MOE (Ministry of Education), Huazhong Agricultural University, Wuhan, China.

Methods in Enzymology
|July 25, 2022
PubMed
Summary

This chapter details optimized methods for analyzing carotenoids, the key pigments in citrus fruits. It covers extraction, detection, and profiling using chromatography techniques for improved understanding of citrus nutritional value.

Keywords:
CarotenoidCitrusDADHPLCHorticultural cropsMass spectrometryUHPLC-HRMS/MS

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Area of Science:

  • Agricultural Science
  • Food Chemistry
  • Analytical Chemistry

Background:

  • Citrus fruits possess significant global economic and nutritional importance.
  • Carotenoids are the primary pigments in citrus, enhancing visual appeal and nutritional content.
  • The intricate nature of citrus carotenoids presents analytical challenges.

Purpose of the Study:

  • To describe optimized methods for carotenoid extraction, detection, and analysis in citrus.
  • To provide detailed explanations of High-Performance Liquid Chromatography (HPLC) and Ultra-High-Performance Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (UHPLC-HRMS/MS) strategies.
  • To outline the applications, benefits, and drawbacks of these analytical methods for major citrus species.

Main Methods:

  • Carotenoid extraction protocols tailored for citrus matrices.
  • High-Performance Liquid Chromatography (HPLC) for carotenoid separation and quantification.
  • Ultra-High-Performance Liquid Chromatography-High-Resolution Tandem Mass Spectrometry (UHPLC-HRMS/MS) for precise carotenoid profiling.

Main Results:

  • Established optimized procedures for carotenoid analysis in citrus.
  • Detailed methodologies for HPLC and UHPLC-HRMS/MS applied to citrus carotenoids.
  • Comparative overview of analytical method performance for mandarin, orange, and pummelo.

Conclusions:

  • The described methods enable robust carotenoid profiling in citrus.
  • HPLC and UHPLC-HRMS/MS are effective tools for citrus carotenoid analysis.
  • Understanding carotenoid profiles is crucial for citrus quality and nutritional assessment.