Related Experiment Video
Updated: Sep 3, 2025

LED-Based In Vitro Screening for Assessing Photoactivable Molecules in Bacterial Photodynamic Inactivation
Published on: January 24, 2025
Whitening efficacy of low concentration hydrogen peroxide photoactivated with blue or violet LED
Joatan Lucas de Sousa Gomes Costa1, João Felipe Besegato1, Joissi Ferrari Zaniboni1
1Department of Restorative Dentistry, School of Dentistry, Araraquara, São Paulo State University - UNESP, 1680 Humaitá Street - 3rd floor, Centro, Mail Box: 331, ZIP Code 14801-903, Araraquara, São Paulo, Brazil.
Background:
Bleaching protocols using low concentration hydrogen peroxide (HP) photoactivated with LED sources have been widely discussed. Herein, we evaluated the whitening efficacy of 15% HP photoactivated with blue or violet LED compared to 35% HP.
Methods:
Thirty bovine crowns were sectioned into 5 × 5 × 2 mm specimens. After staining in black tea, the specimens were randomized into three groups (n = 10): 35% HP, 15% HP + blue LED and 15% HP + violet LED. Two bleaching sessions were performed and the color assessment (∆L*, ∆a*, ∆b*, ∆Eab [CIELab], ∆E00 [CIEDE2000] and WID) was performed before, 24h after each session, 7 days and 1 month after the last session. Data were evaluated by two-way repeated measures ANOVA and Bonferroni post-test at a significance level of 5%.
Results:
All groups showed effective and similar results over 1 month of follow-up (p > 0.05), with only intragroup differences among the time intervals (p < 0.05).
Conclusions:
The use of 15% HP photoactivated with blue or violet LED showed similar whitening efficacy compared to 35% HP. Thus, the association of low concentration bleaching gels with blue or violet LED sources can provide successful and less-aggressive treatment in terms of color change.
More Related Videos
09:29In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging
Published on: April 28, 2017
08:25Inactivation of Pathogens via Visible-Light Photolysis of Riboflavin-5′-Phosphate
Published on: April 6, 2022