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Updated: Sep 3, 2025

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Human Endothelial Cell Seeding in Partially Decellularized Kidneys.

Geraldine Haeublein¹, Gabriela Lombardi¹, Fiorella Caro¹

¹Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Técnicas, Centro de Estudios Farmacológicos y Botánicos (CEFYBO), Facultad de Medicina, Buenos Aires, Argentina.

Biomed Research International

|July 25, 2022

Summary

Researchers developed a rat-human bioartificial kidney by replacing rat endothelial cells with human ones. This method shows promise for reducing organ graft damage and increasing transplant compatibility.

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Related Experiment Videos

Last Updated: Sep 3, 2025

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Epithelial Cell Repopulation and Preparation of Rodent Extracellular Matrix Scaffolds for Renal Tissue Development

Published on: August 10, 2015

Preparation of Decellularized Kidney Scaffolds in Rats

Preparation of Decellularized Kidney Scaffolds in Rats

Published on: March 18, 2021

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Microdissection of Primary Renal Tissue Segments and Incorporation with Novel Scaffold-free Construct Technology

Published on: March 27, 2018

Area of Science:

Regenerative Medicine
Transplantation Biology
Bioengineering

Background:

High demand for organ transplants necessitates innovative solutions like xenotransplantation and bioartificial organs.
Developing immune-compatible organs is crucial for overcoming transplant limitations.

Purpose of the Study:

To describe a method for creating a rat-human bioartificial kidney by partially replacing rat endothelial cells with human endothelial cells.
To evaluate different solutions for selective endothelial cell decellularization while preserving parenchymal cells.

Main Methods:

Perfusing rat kidneys with 0.1% sodium dodecyl sulfate (SDS), 0.01% SDS, or hyperosmolar sucrose solutions to selectively remove endothelial cells.
Recellularizing partially decellularized kidneys with human endothelial cells.
Transplanting the bioartificial kidneys into rats and assessing perfusion and urine production.

Main Results:

Hyperosmolar solutions and 0.01% SDS achieved partial endothelial decellularization, with hyperosmolar solutions better preserving epithelial cell viability.
Recellularized kidneys demonstrated successful attachment of human endothelial cells throughout the vascular bed.
Post-transplant, the bioartificial kidneys achieved complete perfusion and produced urine for at least 90 minutes.

Conclusions:

Partial endothelial decellularization and recellularization with patient-derived human endothelial cells is a feasible approach for creating bioartificial kidneys.
This method holds potential for reducing damage to organ grafts and improving transplant outcomes.