Related Experiment Video

Updated: Sep 3, 2025

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation
09:56

Intra-tracheal Administration of Haemophilus influenzae in Mouse Models to Study Airway Inflammation

Published on: March 2, 2016

10.4K

Mycoplasma hyopneumoniae Infection Activates the NOD1 Signaling Pathway to Modulate Inflammation

Wei Liu1, Pengcheng Jiang1,2, Keli Yang1

  • 1Key Laboratory of Prevention and Control Agents for Animal Bacteriosis (Ministry of Agriculture and Rural Affairs), Hubei Provincial Key Laboratory of Animal Pathogenic Microbiology, Institute of Animal Husbandry and Veterinary Sciences, Hubei Academy of Agricultural Sciences, Wuhan, China.

Frontiers in Cellular and Infection Microbiology
|July 25, 2022
PubMed

Insights

Mycoplasma hyopneumoniae infection activates the NOD1 pathway, crucial for host defense. NOD1 enhances immune responses and limits bacterial proliferation, offering new insights into porcine enzootic pneumonia.

Area of Science:

  • Immunology
  • Microbiology
  • Veterinary Science

Background:

  • Mycoplasma hyopneumoniae causes porcine enzootic pneumonia, a significant swine respiratory disease.
  • Pattern recognition receptors (PRRs) modulate the inflammatory response to M. hyopneumoniae.
  • The role of nucleotide-binding oligomerization domain 1 (NOD1) in M. hyopneumoniae infection is not well understood.

Purpose of the Study:

  • To investigate the role of NOD1 in host defense against Mycoplasma hyopneumoniae.
  • To elucidate the interaction between M. hyopneumoniae and the NOD1 pathway.

Main Methods:

  • Co-localization studies of M. hyopneumoniae and host NOD1.
  • siRNA knockdown of NOD1 to assess its impact on immune pathways.
  • NOD1 overexpression to evaluate its effect on bacterial proliferation.
  • Investigation of the interaction between M. hyopneumoniae mhp390 and NOD1.

Main Results:

  • Mycoplasma hyopneumoniae activates the NOD1-RIP2 pathway and co-localizes with NOD1.
  • NOD1 knockdown impairs TRIF and MYD88 pathways and blocks TNF-α activation.
  • NOD1 overexpression suppresses M. hyopneumoniae proliferation.
  • The M. hyopneumoniae protein mhp390 and host NOD1 are potentially involved in pathogen recognition.

Conclusions:

  • NOD1 plays a critical role in the host immune response to Mycoplasma hyopneumoniae.
  • NOD1 activation is essential for effective inflammatory signaling and bacterial control.
  • The interaction between mhp390 and NOD1 warrants further investigation for therapeutic targets.
Keywords:
Mycoplasma hyopneumoniaeNOD1inflammationinteraction.mhp390

More Related Videos

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.0K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

10.8K

Related Concept Videos

Pneumonia II: Pathophysiology01:29

Pneumonia II: Pathophysiology

The pathophysiology of pneumonia involves the following steps:
473
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.8K
Chronic Obstructive Pulmonary Disease-II: Pathophysiology01:20

Chronic Obstructive Pulmonary Disease-II: Pathophysiology

Chronic Obstructive Pulmonary Disease (COPD) pathophysiology is intricate and multifaceted, involving a complex interplay of physiological processes. Understanding these mechanisms is crucial for effectively managing and treating COPD. Here is an in-depth look at the critical elements in the pathophysiology of COPD:
Chronic Inflammation
3.0K
COPD: Pathogenesis and Clinical Features01:20

COPD: Pathogenesis and Clinical Features

Chronic obstructive pulmonary disease (COPD) is a group of lung conditions that progressively worsen over time, including chronic bronchitis and emphysema. This cluster of diseases collectively leads to a gradual and irreversible decline in lung function over time.
The primary cause for the onset of COPD is cigarette smoking and exposure to air pollution. These hazardous factors initiate a chain reaction within the lungs, resulting in chronic inflammation, damage to the airways, and a...
488
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.2K
Pulmonary Hypertension: Classification and Pathogenesis01:30

Pulmonary Hypertension: Classification and Pathogenesis

Pulmonary hypertension (PH) is a severe health condition in which the mean pulmonary arterial pressure increases to 25 mmHg or more, even when the body is at rest. This high pressure in the blood vessels that transport blood from the heart to the lungs can cause various symptoms, including shortness of breath, can lead to right heart failure, and significantly affect the overall quality of life.
There are various classifications for PH, each relating to different underlying causes and also...
277