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Author Spotlight: Expanding the Scope of Multiplex Immunoassays for Lyme Borreliosis Diagnostics and Pathogen Research
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Modified Two-Tiered Testing Enzyme Immunoassay Algorithm for Serologic Diagnosis of Lyme Disease
Farhan Khan1, Ziyad Allehebi1, Yahya Shabi1
1Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Science Centre, Halifax, Nova Scotia, Canada.
Open Forum Infectious Diseases
|July 25, 2022
Abstract:
The modified 2-tier testing algorithm (MTTT) for Lyme disease (LD) has been approved by the US Food and Drug Administration. In this study, we show that the MTTT detected 28% more cases of early infection compared with the standard 2-tier algorithm while retaining high specificity in a region with a high incidence of LD.

