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Protocol to establish a stable MLL-AF9_AML mouse model.

Jun Lu1, Huanhuan Zhao1, Lingling Yang1

  • 1Department of Pharmacology and Bone Marrow Transplantation Center of the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China; Liangzhu Laboratory, Zhejiang University Medical Center, 1369 West Wenyi Road, Hangzhou, Zhejiang 311121, China; Institute of Hematology, Zhejiang University & Zhejiang Engineering Laboratory for Stem Cell and Immunotherapy, Hangzhou, Zhejiang 310003, China.

STAR Protocols
|July 25, 2022
PubMed
Summary
This summary is machine-generated.

We developed a stable mouse model for studying acute myeloid leukemia (AML) using MLL-AF9. This optimized model facilitates drug testing and interventions for this fatal hematopoietic malignancy.

Keywords:
CancerCell isolationCell separation/fractionationCell-based Assays

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Area of Science:

  • Hematology
  • Oncology
  • Mouse Models

Background:

  • Acute myeloid leukemia (AML) is a prevalent and lethal hematopoietic malignancy.
  • Investigating AML pathogenesis and treatment requires robust experimental models.

Purpose of the Study:

  • To establish and optimize a stable mouse model for studying MLL-AF9-driven acute myeloid leukemia.
  • To provide a reliable platform for drug administration and interventions in AML research.

Main Methods:

  • Development of a mouse model using MLL-AF9 retroviral vector.
  • Optimization of retrovirus infection and bone marrow transplantation (BMT) protocols.
  • Implementation of two rounds of BMT to enhance model stability and efficiency.

Main Results:

  • A stable and efficient mouse model for MLL-AF9 AML was successfully created.
  • Optimized protocols ensure reproducibility for leukemia monitoring.
  • The model is amenable to drug administration and other experimental interventions.

Conclusions:

  • The described MLL-AF9 mouse model provides a valuable tool for acute myeloid leukemia research.
  • Optimized protocols enhance the stability and efficiency of AML modeling.
  • This model facilitates preclinical studies and therapeutic development for AML.