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Related Experiment Video

Updated: Sep 3, 2025

In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
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Aptamer-based biosensors for virus protein detection.

Beibei Lou1, Yanfei Liu2, Meilin Shi3

  • 1Department of Pharmaceutics, Xiangya School of Pharmaceutical Sciences, Central South University, Changsha, 410013, Hunan Province, PR China.

Trends in Analytical Chemistry : TRAC
|July 25, 2022
PubMed
Summary
This summary is machine-generated.

Aptasensors offer a promising approach for rapid virus detection. This review details aptasensor mechanisms and viral targets, aiding in developing effective diagnostic tools, especially for SARS-CoV-2.

Keywords:
AptamerAptasensorDetectionVirus

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Area of Science:

  • Biomedical Engineering
  • Molecular Diagnostics
  • Biosensing Technology

Background:

  • Accurate and early virus detection is crucial for effective clinical management and treatment.
  • Aptasensors, utilizing aptamers (DNA/RNA), are emerging as sensitive tools for pathogen identification.
  • The review focuses on aptasensor applications in virology, with a specific emphasis on SARS-CoV-2 detection.

Purpose of the Study:

  • To review and classify aptasensors for virus detection based on viral target proteins.
  • To illustrate common detection mechanisms employed in aptasensors.
  • To summarize aptamers targeting various viral proteins and analyze their performance.

Main Methods:

  • Literature review of recent aptasensor research for virus detection.
  • Classification of aptasensors by viral target protein.
  • Analysis of detection mechanisms including colorimetry, fluorescence, SPR, SERS, electrochemical methods, and FETs.

Main Results:

  • Summarized aptamers against diverse viral target proteins.
  • Detailed common aptasensor detection mechanisms and their performance.
  • Highlighted the relationship between viral biomarkers and detection method efficacy.

Conclusions:

  • Aptasensors provide versatile platforms for virus detection, adaptable to various viral targets and detection principles.
  • The review offers valuable insights for developing on-site aptasensors, particularly for emerging viral threats like SARS-CoV-2.
  • Further research into aptasensor challenges and future directions is essential for advancing rapid diagnostics.