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Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
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Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Related Experiment Video

Updated: Sep 3, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

Quantitative Cryo-Electron Tomography.

Paula P Navarro^1,2

¹Department of Molecular Biology, Massachusetts General Hospital, Boston, MA, United States.

Frontiers in Molecular Biosciences

|July 25, 2022

Summary

This summary is machine-generated.

Cryo-electron tomography visualizes biomolecular organization in cells. Advances in sample thinning and quantitative methods enable imaging larger systems and understanding cellular functions under various conditions.

Keywords:

cell biology cryo-electron tomography image processing in situ structural biology quantitative cryo-electron tomography

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Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

Area of Science:

Structural Biology
Cell Biology
Biophysics

Background:

Understanding the three-dimensional organization of biomolecules is crucial for all living systems.
Cryo-electron tomography (cryo-ET) allows imaging biomolecules within their native cellular environments.
Cryo-ET is evolving with new technologies for sample preparation and data acquisition.

Purpose of the Study:

To review current hardware and software advancements in quantitative cryo-electron tomography.
To discuss the capabilities and limitations of quantitative cryo-ET for studying biological systems.
To explore how overcoming current limitations can enhance the power of cryo-ET.

Main Methods:

Advancements in sample thinning techniques for cryo-electron tomography.
Development of new hardware and software for quantitative cryo-ET data acquisition and analysis.
Imaging of increasingly large and complex biological systems at multiple scales.

Main Results:

Quantitative cryo-ET can reveal the impact of molecular and environmental changes on subcellular structures.
New methods enable visualization of larger and more complex biological systems.
Current technologies are expanding the scope and resolution of cryo-ET studies.

Conclusions:

Quantitative cryo-electron tomography is a powerful tool for understanding fundamental biological processes.
Overcoming current technological limitations will further unlock the potential of cryo-ET.
Continued development in hardware and software is essential for advancing cryo-ET applications.