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Related Concept Videos

EPS and iPS Cells in Disease Research01:21

EPS and iPS Cells in Disease Research

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Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...
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Related Experiment Video

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Scalable Biomanufacturing Workflow to Produce and Isolate Natural Killer Cell-Derived Extracellular Vesicle-Based Cancer Biotherapeutics
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iPSCs in NK Cell Manufacturing and NKEV Development.

Nicholas Boyd-Gibbins1, Peter Karagiannis2, Do Won Hwang3

  • 1THERABEST Japan, Inc., Kobe, Japan.

Frontiers in Immunology
|July 25, 2022
PubMed
Summary
This summary is machine-generated.

Induced pluripotent stem cells (iPSCs) offer a scalable manufacturing platform for natural killer (NK) cell immunotherapies. This approach also supports the development of NK cell extracellular vesicles (NKEVs) as novel cancer therapeutics.

Keywords:
cancerexosomesextracellular vesiclesgenome engineeringimmunotherapyinduced pluripotent stem cellsmanufacturingnatural killer cells

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Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells hESCs and Induced Pluripotent Stem Cells iPSCs
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Area of Science:

  • Immunology
  • Biotechnology
  • Cancer Therapy

Background:

  • Natural killer (NK) cell immunotherapies are emerging as a complement to T cell-based treatments.
  • Induced pluripotent stem cells (iPSCs) provide a consistent source for NK cell manufacturing and genetic modification.
  • Extracellular vesicles (EVs), specifically NK cell extracellular vesicles (NKEVs), are gaining attention for their therapeutic potential.

Purpose of the Study:

  • To review the application of induced pluripotent stem cell (iPSC) technology in natural killer (NK) cell manufacturing.
  • To explore the potential of iPSC-derived NK cells and NK cell extracellular vesicles (NKEVs) as cancer therapeutics.
  • To highlight the advantages of iPSCs for engineering NK cells and NKEVs.

Main Methods:

  • Literature review of iPSC technology in NK cell therapy.
  • Analysis of research on NK cell extracellular vesicles (NKEVs) and their functions.
  • Exploration of iPSC-based platforms for NK cell and NKEV development.

Main Results:

  • iPSCs offer a renewable and genetically engineerable source for NK cell production, overcoming donor variability.
  • NK cell extracellular vesicles (NKEVs) derived from parent NK cells can replicate their therapeutic functions.
  • NKEVs present a potential cell-free therapeutic alternative with reduced immunogenicity and complexity compared to cell-based therapies.

Conclusions:

  • iPSC technology is pivotal for advancing NK cell manufacturing and enabling the development of NKEV therapeutics.
  • The combination of iPSCs and NKEVs holds significant promise for next-generation cancer immunotherapy.
  • Further research into iPSC-derived NKEVs could lead to innovative and safer cancer treatments.