Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.2K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.2K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.2K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Addendum: In situ architecture of the human prohibitin complex.

Nature cell biology·2026
Same author

A versatile nanobody platform for live and super-resolution imaging of synaptic vesicle dynamics and plasticity in rodent and human neurons.

Journal of nanobiotechnology·2026
Same author

Dual inhibition of PP2A and WEE1 induces apoptosis and mitotic catastrophe in cancer cells.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Pluripotent stem-cell-based screening uncovers sildenafil as a mitochondrial disease therapy.

Cell·2026
Same author

Comparison of the Biomechanical Stability of Two Fix-and-Replace Techniques in an Acetabular Fracture Model with Pelvic Discontinuity.

Journal of clinical medicine·2026
Same author

HIF sustain a transcriptional regulatory circuit of EPAS1 expression in renal clear cell carcinoma.

Nature communications·2026

Related Experiment Video

Updated: Sep 3, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K

Colocalization for super-resolution microscopy via optimal transport.

Carla Tameling1, Stefan Stoldt2,3, Till Stephan2,3

  • 1Institute for Mathematical Stochastics, University of Göttingen, Göttingen, Germany.

Nature Computational Science
|July 25, 2022
PubMed
Summary
This summary is machine-generated.

New optimal transport colocalization methods accurately analyze protein distributions in super-resolution microscopy images. This technique addresses challenges posed by high-resolution Stimulated Emission Depletion (STED) microscopy data.

More Related Videos

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.3K
Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.3K

Related Experiment Videos

Last Updated: Sep 3, 2025

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells
11:06

Multi-color Localization Microscopy of Single Membrane Proteins in Organelles of Live Mammalian Cells

Published on: June 30, 2018

8.6K
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
10:41

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Published on: May 19, 2022

2.3K
Ground State Depletion Super-resolution Imaging in Mammalian Cells
07:55

Ground State Depletion Super-resolution Imaging in Mammalian Cells

Published on: November 5, 2017

7.3K

Area of Science:

  • Cell Biology
  • Microscopy
  • Biophysics

Background:

  • Super-resolution fluorescence microscopy, including Stimulated Emission Depletion (STED) microscopy, is crucial for visualizing cellular structures at subdiffraction resolutions.
  • High-resolution imaging presents new challenges for analyzing the colocalization of macromolecules, as traditional methods may not be suitable.
  • Accurate colocalization analysis is essential for understanding molecular interactions and cellular organization.

Purpose of the Study:

  • To evaluate the suitability of existing colocalization analysis methods for high-resolution STED microscopy data.
  • To introduce and validate a novel colocalization method, optimal transport colocalization, for super-resolution microscopy.
  • To extend the optimal transport colocalization methodology to other nanoscopy techniques like coordinate-stochastic nanoscopy.

Main Methods:

  • Comparison of established colocalization analysis techniques with optimal transport colocalization on simulated and experimental datasets.
  • Application of optimal transport colocalization to dual-color STED microscopy images of yeast and mammalian cells.
  • Extension of the optimal transport colocalization framework for use with coordinate-stochastic nanoscopy data.

Main Results:

  • Traditional colocalization methods are not optimal for analyzing high-resolution STED microscopy data.
  • Optimal transport colocalization effectively measures protein intensity distribution matching below a defined spatial scale.
  • The proposed method demonstrates validity on simulated data and experimental STED recordings from cellular samples.

Conclusions:

  • Optimal transport colocalization offers a robust solution for analyzing macromolecule distributions in high-resolution STED microscopy.
  • The developed methodology enhances the accuracy of colocalization analysis in super-resolution imaging.
  • This approach is adaptable and extends to other advanced microscopy techniques, broadening its applicability in cell biology research.