Lanthanide/Cu_2-Se Nanoparticles for Bacteria-Activated NIR-II Fluorescence Imaging of Infection

A material system enabling specific NIR-II fluorescence imaging of Gram-positive bacteria is described. The material system is based on the electrostatic binding of Cu_2-Se and vancomycin-modified NaGdF₄:Nd,Yb@NaGdF₄ downconversion nanoparticles (DCNPs), the fluorescence of which is weak owing to the spectral overlap of Cu_2-Se absorption with the DCNP NIR emission. The presence of Gram-positive bacteria precisely disconnects the bond between vancomycin-modified DCNPs and Cu_2-Se, thus enabling a strong fluorescent signal. In vivo studies show that the material system can be specifically activated at the site of Gram-positive bacterial infection but is essentially nonfluorescent in the area of Gram-negative bacterial infection.