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Updated: Aug 16, 2026
09:27
Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
Diploid formation of Candida tropicalis via protoplast fusion
Acta Microbiologica Academiae Scientiarum Hungaricae
|January 1, 1978
Abstract:
Haploid auxotrophic mutants were produced from Candida tropicalis, and protoplast usion was induced by polyethylene glycol. The resulting nutritional complementation was due to heterokaryon formation and, at a much lower frequenty, to spontaneous diploidization. During cultivation, heterokaryotic clones regularly gave rise to heterozygous diploids from which, in turn, haploids could be isolated. The technique of protoplast fusion gives an opportunity for genetic analysis of this and similarly asexual fungal species.
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