Cellular diversity and developmental hierarchy in the planarian nervous system

Our ability to dissect cell type diversity, development, and plasticity in the nervous system has been transformed by the recent surge of massive sequencing studies at the single-cell level. A large body of this work has focused primarily on organisms with nervous systems established early in development. Using planarian flatworms in which neurons are constantly respecified, replenished, and regenerated, we analyze several existing single-cell transcriptomic datasets and observe features in neuron identity, differentiation, maturation, and function that may provide the planarian nervous system with high levels of adaptability required to respond to various cues including injury. This analysis allows us to place many prior observations made by functional characterizations in a general framework and provide additional hypothesis and predictions to test in future investigations.