Related Experiment Video
Updated: Sep 21, 2026

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Valorization of brewer's spent grain by consecutive supercritical carbon dioxide extraction and enzymatic hydrolysis
Patricia Alonso-Riaño1, Rodrigo Melgosa1, Ester Trigueros1
1Department of Biotechnology and Food Science, University of Burgos, Plaza Misael Bañuelos s/n, 09001 Burgos, Spain.
Abstract:
The double effect of supercritical carbon dioxide, sc-CO2, in a biorefinery concept applied to brewer's spent grain (BSG) was assessed in this work. Extraction conditions to remove and valorize the lipophilic fraction were studied (20-40 MPa and 40-80 °C) obtaining a maximum yield of 5.70 ± 0.07 g/100 gBSG at 80 °C and 40 MPa. High pressures and temperatures resulted in higher content of total phenolic and flavonoid compounds, as well as higher antioxidant capacity. It was observed an improvement of the enzymatic hydrolysis yield by cellulase in the sc-CO2 treated BSG compared to the non-treated. This improvement could be partially attributed to the removal of the lipid fraction and to morphological changes of BSG after sc-CO2. Based on this double benefit, sc-CO2 can play an important role on biomass valorization.
More Related Videos
14:53Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
06:08Design of Solid-State Fermentation Systems for Polymer Hydrolytic Extracellular Enzyme Production by Filamentous Fungi
Published on: June 6, 2025
Related Concept Videos
Microbes in Beverage Production
Bioreactor Controls-III
Production of Alcohol
Production of Organic Acids
Biofuels
Downstream Processing