A hemicyanidin-based NIR fluorescent probe for detection of H₂S and imaging study in cells and mice

The selective detection of hydrogen sulfide in physiological and pathological processes has gained substantial attention in recent years. However, the real-time detection of hydrogen sulfide remains an elusive goal. In this work, a new type of hemicyanidin-based fluorescent "turn-on" probe NTR-HS (Ex = 680 nm, Em = 760 nm) was developed to detected H₂S in a very short time (3 min). The fluorescence quantum yield is 0.15 and accompanied with a noticeable color change from violet to blue that can be used to detect H₂S in the range 1.04 × 10^-7-4 × 10^-5 M with a limit of detection of 1.04 x 10^-7 M. The NTR-HS probe was also used for imaging of endogenous hydrogen sulfide and mitochondrial localization in HCT116 and HeLa cells. The detection mechanism was studied through fluorescence, UV-Vis, NMR, and mass analysis. Notably, the probe was successfully used to imaging H₂S in mice and locating hydrogen sulfide in the large intestine of mice.