Dual photothermal nanocomposites for drug-resistant infectious wound management

Management of antibiotic-resistant bacteria-induced skin infections for rapid healing remains a critical clinical challenge. Photothermal therapy, which uses mediated hyperthermia to combat such problems, has recently been recognised as a promising approach to take. In this study, bacterial cellulose-based photothermal membranes were designed and developed to combat bacterial infections and promote rapid wound healing. Polydopamine was incorporated into gold nanoparticles to produce superior dual-photothermal behaviour. The in vitro antibacterial efficacy of the prepared composite membranes against S. aureus, E. coli and methicillin-resistant Staphylococcus aureus (MRSA) could reach 99% under near-infrared (NIR) irradiation. In addition, the synthesised nanocomposite exhibited good biocompatibility in vitro as demonstrated by a cell survival ratio of >85%. The effectiveness of the composite membranes on wound healing was further investigated in a murine model of MRSA-infected wounds, focusing on the effect of photothermal temperature. According to the detailed therapeutic mechanism study undertaken, the composite membranes cause bacterial killing initially and promote the transition from the inflammatory phase to proliferation by suppressing pro-inflammatory cytokine production, promoting collagen deposition, and stimulating angiogenesis. Considering their remarkable effectiveness and facile fabrication process, it is expected that these novel materials could serve as competitive multifunctional dressings in the management of infectious wounds and accelerate the regeneration of damaged tissues related to abnormal immune responses.