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Biocompatible silane adhesion layer on titanium implants improves angiogenesis and osteogenesis.

Yuyu Zhao1, Yonghua Sun1, Ruiyue Hang1

  • 1Shanxi Key Laboratory of Biomedical Metal Materials, College of Materials Science and Engineering, Taiyuan University of Technology, Taiyuan 030024, China.

Biomaterials Advances
|July 26, 2022
PubMed
Summary

Optimizing silanization time for titanium implants enhances biocompatibility and bioactivity. A 12-hour silanization process promotes endothelial cell and osteoblast function, improving osseointegration through enhanced angiogenesis and osteogenesis.

Keywords:
Adhesion layerAngiogenesisOsteogenesisSilanizationTitanium

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Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Tissue Engineering

Background:

  • Silane adhesion layers are crucial for covalently grafting biomolecules to titanium implants, enhancing osseointegration.
  • The impact of silanization parameters on the biocompatibility and biofunctionality of these layers remains under-explored.

Purpose of the Study:

  • To investigate the effects of varying silanization parameters on the properties of silane adhesion layers on titanium.
  • To evaluate the in vitro impact of these modified layers on angiogenesis and osteogenesis.

Main Methods:

  • Fabrication of silane adhesion layers using 3-aminopropyltriethoxysilane on alkali-treated titanium with varied silanization times.
  • Assessment of layer thickness, intactness, and surface morphology.
  • In vitro evaluation of endothelial cell (EC) and osteoblast (OB) responses, including proliferation, VEGF and nitric oxide (NO) production, alkaline phosphatase (ALP) activity, collagen secretion, and extracellular matrix mineralization.

Main Results:

  • Prolonged silanization increased layer thickness and intactness, significantly improving biocompatibility.
  • A 12-hour silanization resulted in a unique brain-like surface morphology, promoting EC and OB adhesion and proliferation.
  • The modified layers enhanced angiogenesis via increased VEGF and NO secretion by ECs.
  • Osteogenesis was improved, evidenced by enhanced ALP activity, collagen secretion, and matrix mineralization in OBs.

Conclusions:

  • Silanization processing time is a critical factor in tailoring the biocompatibility and biofunctionality of silane adhesion layers on titanium.
  • The 12-hour silanized layer demonstrates significant potential for enhancing osseointegration by promoting both angiogenesis and osteogenesis.
  • This study provides valuable insights for optimizing silane-based surface modifications for titanium implants.