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TRPM7 Modulates Human Pancreatic Stellate Cell Activation
Julie Auwercx1, Philippe Kischel1, Thibaut Lefebvre1
1UR-UPJV 4667, UFR des Sciences, Université de Picardie Jules Verne, F-80039 Amiens, France.
Cells
|July 27, 2022
Summary
TRPM7 channels are crucial in pancreatic stellate cell (PSC) activation and proliferation. Targeting TRPM7 may offer a novel therapeutic strategy for pancreatic diseases by modulating PSCs.
Area of Science:
- Cell Biology
- Oncology
- Gastroenterology
Background:
- Pancreatic diseases involve activated pancreatic stellate cells (PSCs), key players in tumor stroma.
- Reprogramming PSCs to a quiescent state is a potential therapeutic strategy.
Purpose of the Study:
- To investigate the role of Transient Receptor Potential Melastatin 7 (TRPM7) channels in PSC activation and proliferation.
- To determine if TRPM7 can serve as a marker for activated PSCs.
Main Methods:
- Used PS-1 cells as a model for healthy PSCs, inducing quiescence or activation.
- Employed RNA silencing and pharmacological inhibition to study TRPM7 function.
- Analyzed TRPM7 expression correlation with PSC activation status and proliferation.
Main Results:
- TRPM7 expression levels correlated with PSC activation status.
- TRPM7 regulated PSC proliferation via cell cycle regulators, including p53, through the PI3K/Akt pathway in a magnesium-dependent manner.
- TRPM7 was overexpressed in cancer-associated fibroblasts in TCGA database.
Conclusions:
- TRPM7 plays a significant role in PSC activation and proliferation.
- TRPM7 can be considered a reliable marker for activated PSCs in pancreatic diseases.
- TRPM7 inhibition represents a potential therapeutic target for pancreatic diseases.
