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The Array of Antibacterial Action of Protocatechuic Acid Ethyl Ester and Erythromycin on Staphylococcal Strains

Maria Miklasińska-Majdanik1, Małgorzata Kępa1, Monika Kulczak1

  • 1Department of Microbiology and Virology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Jagiellońska 4, 41-200 Sosnowiec, Poland.

Antibiotics (Basel, Switzerland)
|July 27, 2022
PubMed

Insights

This study explored combining protocatechuic acid ethyl ester with erythromycin to combat antibiotic-resistant bacteria. The combination showed synergistic effects against some multi-drug-resistant staphylococcal strains, offering a potential new strategy.

Area of Science:

  • Microbiology
  • Pharmacology
  • Infectious Diseases

Background:

  • Antibiotic resistance, particularly from methicillin-resistant staphylococcal strains, is a global health crisis.
  • Resistance to macrolides, lincosamides, and streptogramin B (MLSB) limits treatment options for staphylococcal infections.
  • Combining natural compounds with antibiotics presents a promising strategy against multi-drug-resistant pathogens.

Purpose of the Study:

  • To evaluate the antibacterial activity of protocatechuic acid ethyl ester combined with erythromycin.
  • To assess this combination against Staphylococcus aureus and Staphylococcus epidermidis strains with diverse resistance profiles.
  • To investigate the synergistic, additive, or indifferent effects of the combination on bacterial growth.

Main Methods:

  • In vitro antibacterial assays were performed.
  • Minimum inhibitory concentration (MIC) determination was used.
  • Checkerboard testing was employed to assess drug interactions.

Main Results:

  • The combination's effect was strain-dependent.
  • Synergistic effects were observed in 2 out of 12 tested staphylococcal strains.
  • Multi-drug-resistant strains exhibited increased sensitivity to the protocatechuic acid ethyl ester-erythromycin combination.

Conclusions:

  • Protocatechuic acid ethyl ester-erythromycin combination demonstrates potential as an alternative therapeutic approach.
  • The combination shows promise in combating multi-drug-resistant staphylococcal infections.
  • Further research into natural compound-antibiotic combinations is warranted.
Keywords:
Staphylococcus spp.erythromycinfractional inhibitory concentrationprotocatechuic acid ethyl ester

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