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APTES-Modified Remote Self-Assembled DNA-Based Electrochemical Biosensor for Human Papillomavirus DNA Detection
Yuxing Yang1, Yang Qing1, Xudong Hao1
1College of Materials Science and Engineering, Hainan University, Haikou 570228, China.
Biosensors
|July 27, 2022
Summary
A novel biosensor detects high-risk human papillomavirus (HPV) DNA, crucial for preventing cervical cancer. This sensitive and stable electrochemical sensor offers early disease detection and diagnosis.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Oncology
Background:
- High-risk human papillomavirus (HPV) infection is a primary cause of cervical cancer.
- Early detection of high-risk HPV, such as HPV-16, is vital for timely treatment and prevention strategies.
- Existing diagnostic methods require improvement in sensitivity and stability.
Purpose of the Study:
- To develop a highly sensitive and stable electrochemical biosensor for detecting high-risk HPV-16 DNA.
- To establish a potential clinical tool for early cervical cancer diagnosis and disease management.
Main Methods:
- Fabrication of a biosensor using a 3-aminopropyltriethoxysilane (APTES) modified gold electrode.
- Implementation of a super sandwich structure for enhanced target DNA detection.
- Electrochemical analysis to determine sensor performance, including limit of detection and linear range.
Main Results:
- The developed electrochemical biosensor demonstrated a low limit of detection (LOD) of 5.475 × 10-16 mol/L for HPV-16 DNA.
- A wide linear range (1.0 × 10-13 mol/L to 1.0 × 10-6 mol/L) with high linearity (R2 = 0.9923) was achieved.
- The sensor exhibited excellent stability over 7 days in a low-temperature environment and good interference resistance in human serum samples.
Conclusions:
- The novel APTES-modified gold electrode biosensor with a super sandwich structure provides a sensitive and stable platform for HPV-16 detection.
- This biosensor shows significant potential as a clinical tool for early cervical cancer diagnosis due to its performance in complex biological samples.
