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Electrochemical Biosensors Based on Convectively Assembled Colloidal Crystals
Amane Shiohara1,2,3, Christopher D Easton2, Beatriz Prieto-Simon4,5
1Drug Delivery, Deposition, and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Melbourne, VIC 3052, Australia.
Biosensors
|July 27, 2022
Summary
This study presents a novel method for detecting intact viruses using tuneable nanochannels. The biosensor offers sensitive, label-free virus detection for potential point-of-care applications.
Area of Science:
- Nanotechnology
- Biosensing
- Virology
Background:
- Rapid and sensitive virus detection is crucial globally.
- Distinguishing between infectious and non-infectious viral particles remains a challenge.
Purpose of the Study:
- To develop a tuneable nanochannel biosensor for size-selective detection of intact viruses.
- To achieve sensitive, label-free detection of viruses for point-of-care applications.
Main Methods:
- Fabrication of tuneable nanochannels using polystyrene spheres on gold electrodes.
- Utilizing selective nanochannel blockage of a redox probe for detection.
- Surface passivation with silica glancing angle deposition to confine bioreceptor immobilization.
Main Results:
- Optimized detection with biotinylated gold nanoparticles achieved a limit of detection of 37 particles/mL.
- Demonstrated label-free detection of MS2 bacteriophage with a limit of detection of ~1.0 plaque-forming unit/mL.
- The system provides quantifiable current changes based on virus binding.
Conclusions:
- Tuneable nanochannel biosensors offer a sensitive, label-free, and cost-efficient platform for virus detection.
- This technology has potential applications in point-of-care diagnostics for various viruses.
- The method effectively differentiates intact viruses based on size selectivity.
