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Related Experiment Video

Updated: Sep 3, 2025

Phthalic Acid Ester-Binding DNA Aptamer Selection, Characterization, and Application to an Electrochemical Aptasensor
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Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor.

Anabel Villalonga1, Concepción Parrado1, Raúl Díaz1

  • 1Nanosensors and Nanomachines Group, Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, 280409 Madrid, Spain.

Biosensors
|July 27, 2022
PubMed
Summary

This study introduces a new enzymatic labeling method for electrochemical aptasensors using host-guest interactions. The novel aptasensor effectively detects carcinoembryonic antigen in human serum with high sensitivity and selectivity.

Keywords:
aptamerbiosensorcarcinoembryonic antigencyclodextringold nanostars

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Area of Science:

  • Biomedical Engineering
  • Biosensors
  • Nanomaterials

Background:

  • Electrochemical aptasensors offer sensitive detection of biomarkers.
  • Novel labeling strategies are crucial for enhancing aptasensor performance.
  • Supramolecular chemistry provides unique tools for molecular recognition and assembly.

Purpose of the Study:

  • To develop a novel enzymatic labeling strategy for electrochemical aptasensors.
  • To utilize supramolecular host-guest interactions for enhanced bioreceptor and label conjugation.
  • To demonstrate the efficacy of the developed aptasensor for carcinoembryonic antigen detection.

Main Methods:

  • Designed an adamantane-modified DNA aptamer as a target-responsive bioreceptor.
  • Synthesized a β-cyclodextrin (CD) neoglycoconjugate attached to a redox enzyme (horseradish peroxidase) as a labeling element.
  • Fabricated an amperometric aptasensor on gold nanoparticles/graphene oxide modified screen-printed carbon electrodes.

Main Results:

  • The aptasensor successfully detected carcinoembryonic antigen using the enzymatic labeling strategy.
  • Achieved a wide detection range from 10 pg/mL to 1 ng/mL.
  • Demonstrated a low limit of detection of 3.1 pg/mL with high selectivity in human serum samples.

Conclusions:

  • The proposed enzymatic marking strategy based on supramolecular host-guest interactions is effective for electrochemical aptasensors.
  • This novel approach significantly improves sensitivity and selectivity for biomarker detection.
  • The developed aptasensor shows great potential for clinical diagnostics, particularly for carcinoembryonic antigen monitoring.