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The Blood-brain Barrier00:49

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Physiological barriers are semi-permeable cellular structures restricting drug diffusion into intracellular compartments and tissues. There are six types of physiological barriers: blood endothelial, cell membrane, blood-brain, blood-cerebrospinal fluid (CSF), blood-placenta, and blood-testis barriers.
The blood endothelial barrier is the most porous of these. It allows all small ionized, un-ionized, and lipophilic molecules to pass through the endothelial lining into the interstitial space...
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Related Experiment Video

Updated: Sep 3, 2025

Assessment of Morphine-induced Hyperalgesia and Analgesic Tolerance in Mice Using Thermal and Mechanical Nociceptive Modalities
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Chronic Low Dose Morphine Does Not Alter Two In Vitro BBB Models.

Jamie Marino1,2, Monique E Maubert1,2, Jill M Lawrence1,2

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|July 27, 2022
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Repeat morphine dosing did not significantly alter blood-brain barrier (BBB) integrity in human co-culture or cell line models. These findings suggest morphine may not impact BBB function in patients with central nervous system (CNS) diseases.

Keywords:
blood–brain barrier (BBB)morphinetransmigration

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Area of Science:

  • Neuroscience
  • Pharmacology
  • Immunology

Background:

  • The blood-brain barrier (BBB) regulates CNS and peripheral circulation passage.
  • Compromised BBB integrity is associated with neurocognitive deficits in diseases like HIV-1 infection.
  • Understanding pharmaceutical effects on BBB function is crucial for CNS disease management.

Purpose of the Study:

  • To investigate the impact of repeat morphine exposure on BBB integrity.
  • To model BBB systems and assess alterations in permeability, immune cell transmigration, and chemokine gradients.

Main Methods:

  • Two BBB models were utilized: a primary human co-culture and a cell line monoculture.
  • Both models were exposed to three daily repeat doses of morphine.
  • BBB integrity was assessed by measuring permeability, PBMC transmigration, and chemokine gradients.

Main Results:

  • No significant alterations in BBB integrity were observed in either model system following repeat morphine dosing.
  • Morphine exposure did not affect BBB permeability or PBMC transmigration.
  • Chemokine gradient changes were not significantly altered by repeat morphine exposure.

Conclusions:

  • Repeat morphine exposure does not appear to significantly impact blood-brain barrier integrity.
  • These findings suggest that morphine may not play a significant role in BBB dysfunction in the context of CNS diseases.