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Updated: Sep 3, 2025
Localization of Odorant Receptor Genes in Locust Antennae by RNA In Situ Hybridization
Published on: July 13, 2017
Expression Profiling and Functional Analysis of Candidate Odorant Receptors in Galeruca daurica
Jing-Hang Zhang1, Ling Li1, Na Li2
1Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010018, China.
Abstract:
Galeruca daurica (Joannis) is an oligophagous pest in the grasslands of Inner Mongolia, China, which feed mainly on Allium spp. Odorant receptors (ORs) play an important role in the olfactory system in insects, and function together with olfactory co-receptor (ORco). In this study, 21 OR genes were identified from the transcriptome database of G. daurica adults, and named GdauOR1-20 and GdauORco. The expression profiles were examined by RT-qPCR and RNA interference (RNAi) and electroantennogram (EAG) experiments were conducted to further identify the olfactory functions of GdauOR4, GdauOR11, GdauOR15, and GdauORco. It was found that 15 GdauORs (OR1, OR3-6, OR8, OR11-13, OR15, OR17-20, and ORco) were mainly expressed in antennae, and the expression levels of GdauORs in adults were affected by age. When GdauOR4, GdauOR15, and GdauORco were silenced by RNAi, the electrophysiological responses to host plant volatiles were significantly decreased in G. daurica. This study lays a necessary foundation for clarifying the mechanism on finding host plants in G. daurica.
