Related Experiment Video

Updated: Sep 3, 2025

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein
09:39

Co-immunoprecipitation of the Mouse Mx1 Protein with the Influenza A Virus Nucleoprotein

Published on: April 21, 2015

17.9K

Potential Proteins Interactions with Bombyx mori Nucleopolyhedrovirus Revealed by Co-Immunoprecipitation

Xiong Wang1, Guangyu Ma1, Feifei Ren1

  • 1Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China.

Insects
|July 27, 2022
PubMed

Insights

This study identified host proteins interacting with Bombyx mori nucleopolyhedrovirus (BmNPV). SEC61 and PIC promote BmNPV replication, while FABP1 inhibits it, offering insights into virus-host interactions.

Area of Science:

  • Virology
  • Insect Pathology
  • Molecular Biology

Background:

  • Virus-host interactions are crucial for viral replication, virulence, and pathogenicity.
  • Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen in sericulture, impacting silkworm production.
  • Understanding BmNPV's interaction with its host is vital for developing control strategies.

Purpose of the Study:

  • To identify host proteins that interact with Bombyx mori nucleopolyhedrovirus (BmNPV).
  • To investigate the role of identified proteins in BmNPV replication.
  • To provide a foundation for discovering BmNPV receptor proteins.

Main Methods:

  • Construction of a recombinant BmNPV virus particle (rBmBV-egfp-p64-3×flag-gp64sp) using the MultiBac system.
  • Co-immunoprecipitation assays using BmN cells and Dazao silkworms infected with the recombinant virus.
  • Liquid chromatography-tandem mass spectrometry (LC-MS/MS) for protein identification, followed by bioinformatics analysis (Gene Ontology, KEGG Pathway).

Main Results:

  • Identified 845 candidate interacting proteins in BmN cells and 1368 in silkworm samples.
  • Bioinformatics analysis highlighted significantly enriched proteins involved in BmNPV replication.
  • SEC61 and PIC were found to promote BmNPV replication, whereas FABP1 inhibited it.

Conclusions:

  • This study successfully identified potential host proteins involved in BmNPV invasion and proliferation.
  • The findings reveal specific host factors (SEC61, PIC, FABP1) that modulate BmNPV replication.
  • The research establishes a platform for further identification of BmNPV receptor proteins and understanding virus-host dynamics.
Keywords:
Bombyx morico-immunoprecipitationnuclear polyhedrosis virusviral proliferation

More Related Videos

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay10:05

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Published on: January 16, 2017

12.9K
Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro08:38

Protein Membrane Overlay Assay: A Protocol to Test Interaction Between Soluble and Insoluble Proteins in vitro

Published on: August 14, 2011

22.2K

Related Concept Videos

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
Chromatin Immunoprecipitation
Chromatin immunoprecipitation, also known as ChIP, is used to study protein-DNA or...
5.6K
Globular and Fibrous Proteins02:21

Globular and Fibrous Proteins

Many proteins can be classified into two distinct subtypes - globular or fibrous. These two types differ in their shapes and solubilities.
Globular proteins are also known as spheroproteins and typically are approximately round in shape. They contain a mix of amino acid types and contain differing sequences in their primary structures. Globular proteins have many different functions, such as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be...
44.2K
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
4.1K
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
13.0K