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Updated: Sep 3, 2025

Novel Sequence Discovery by Subtractive Genomics
Published on: January 25, 2019
Genomic Characteristics and Comparative Genomics Analysis of Parafenestella ontariensis sp. nov
Evgeny Ilyukhin1, Svetlana Markovskaja2, Abdallah M Elgorban3
1Department of Biological Sciences, Brock University, St. Catharines, ON L2S 3A1, Canada.
Abstract:
A new ascomycetous species of Parafenestella was isolated from Acer negundo during the survey of diseased trees in Southern Ontario, Canada. The species is morphologically similar to other taxa of Cucurbitariacea (Pleosporales). The new species is different from the extant species in the morphology of ascospores, culture characteristics and molecular data. The novel species is described as Parafenestella ontariensis sp. nov. based on morphological and multi-gene phylogenetic analyses using a combined set of ITS, LSU, tef1 and tub2 loci. Additionally, the genome of P. ontariensis was sequenced and analyzed. The phylogenomic analysis confirmed the close relationship of the species to the fenestelloid clades of Cucurbitariaceae. The comparative genomics analysis revealed that the species lifestyle appears to be multitrophic (necrotrophic or hemi-biotrophic) with a capability to turn pathogenic on a corresponding plant host.
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