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Updated: Sep 3, 2025
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Protein Engineering by Yeast Surface Display
Published on: November 29, 2024
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Construction of HGF-Displaying Yeast by Cell Surface Engineering.
Seiji Shibasaki1,2, Yuki Nakatani3, Kazuaki Taketani3
1Laboratory of Natural Science, Faculty of Economics, Toyo University, Hakusan, Bunkyo-ku, Tokyo 112-8606, Japan.
Microorganisms
|July 27, 2022
Summary
Researchers developed a novel method to display Hepatocyte Growth Factor (HGF) on yeast cell surfaces for potential oral administration. This innovation could offer a new way to treat graft-versus-host disease (GVHD).
Area of Science:
- Immunology
- Biotechnology
- Molecular Biology
Background:
- Hepatocyte Growth Factor (HGF) is a key regulator of immune responses, with potential applications in transplantation and autoimmune diseases.
- Previous research indicated that administering HGF via cDNA could mitigate acute graft-versus-host disease (GVHD) following hematopoietic stem cell transplantation.
Purpose of the Study:
- To engineer yeast cells for surface display of HGF protein.
- To explore a novel oral administration strategy for HGF using yeast cell surface display technology.
- To evaluate the efficacy of this approach in a GVHD mouse model.
Main Methods:
- Genetic fusion of full-length HGF and HGF heavy chain with α-agglutinin.
- Display of engineered HGF proteins on the surface of yeast cells.
- Development of a yeast cell-based delivery system for HGF.
Main Results:
- Successful display of full-length HGF and HGF heavy chain on the yeast cell surface was achieved.
- The engineered yeast cells present a potential platform for oral HGF delivery.
- This method offers a novel approach for HGF administration in a GVHD context.
Conclusions:
- Yeast cell surface display engineering is a viable strategy for presenting functional HGF.
- This technology provides a promising new route for the oral administration of HGF.
- The developed system holds potential for managing immune-related conditions like GVHD.
