A Quassinoid Diterpenoid Eurycomanone from Eurycoma longifolia Jack Exerts Anti-Cancer Effect through Autophagy
Guiqin Ye1,2, Mengting Xu2,3, Yuhan Shu2,3
1Hangzhou Medical College, Hangzhou 310014, China.
Molecules (Basel, Switzerland)
|July 27, 2022
Summary
Eurycomanone (EN) inhibits colon cancer growth by suppressing autophagy and angiogenesis. Autophagy inhibition enhances EN
Area of Science:
- Pharmacology
- Molecular Biology
- Cancer Research
Background:
- Eurycomanone (EN), a quassinoid diterpenoid from *Eurycoma longifolia* Jack, shows anti-cancer properties.
- The precise molecular mechanisms underlying EN's anti-cancer effects, particularly its impact on autophagy, remain unclear.
Purpose of the Study:
- To investigate the regulatory role of EN on autophagy in colon cancer.
- To elucidate the mechanism of EN-mediated colon cancer growth inhibition.
Main Methods:
- Assessed EN's effects on colon cancer cell proliferation, colony formation, and angiogenesis.
- Analyzed autophagy suppression via LC3-II levels and GFP-LC3 puncta formation.
- Investigated the mTOR signaling pathway activation in response to EN treatment.
Main Results:
- EN significantly inhibited colon cancer cell proliferation, colony formation, and angiogenesis.
- EN treatment suppressed autophagy, evidenced by reduced LC3-II levels and GFP-LC3 puncta.
- EN activated the mTOR signaling pathway in a time- and concentration-dependent manner.
- Autophagy induction was found to promote colon cancer cell survival against EN treatment.
Conclusions:
- EN exerts anti-colon cancer effects by inhibiting autophagy and angiogenesis.
- EN's mechanism involves mTOR pathway activation and autophagy suppression.
- EN demonstrates potential as a novel autophagy inhibitor for anti-cancer therapy.
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