SiO₂ Fibers of Two Lengths and Their Effect on Cellular Responses of Macrophage-like Cells

The immunoreactivity or/and stress response can be induced by nanomaterials' different properties, such as size, shape, etc. These effects are, however, not yet fully understood. This study aimed to clarify the effects of SiO₂ nanofibers (SiO₂NFs) on the cellular responses of THP-1-derived macrophage-like cells. The effects of SiO₂NFs with different lengths on reactive oxygen species (ROS) and pro-inflammatory cytokines TNF-α and IL-1β in THP-1 cells were evaluated. From the two tested lengths, it was only the L-SiO₂NFs with a length ≈ 44 ± 22 µm that could induce ROS. Compared to this, only S-SiO₂NFs with a length ≈ 14 ± 17 µm could enhance TNF-α and IL-1β expression. Our results suggested that L-SiO₂NFs disassembled by THP-1 cells produced ROS and that the inflammatory reaction was induced by the uptake of S-SiO₂NFs by THP-1 cells. The F-actin staining results indicated that SiO₂NFs induced cell motility and phagocytosis. There was no difference in cytotoxicity between L- and S-SiO₂NFs. However, our results suggested that the lengths of SiO₂NFs induced different cellular responses.