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Author Spotlight: Efficient Adeno-Associated Virus Isolation for Pre-Clinical Applications
Published on: February 9, 2024
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Continuous Affinity Purification of Adeno-Associated Virus Using Periodic Counter-Current Chromatography
João P Mendes1,2, Magnus Bergman3, Anita Solbrand3
1iBET, Instituto de Biologia Experimental e Tecnológica, Apartado 12, 2780-901 Oeiras, Portugal.
Pharmaceutics
|July 27, 2022
Summary
Continuous affinity capture of adeno-associated virus (AAV) using periodic counter-current chromatography (PCC) significantly boosts productivity. This method achieves high virus recovery, supporting efficient AAV therapy manufacturing.
Area of Science:
- Biopharmaceutical manufacturing
- Process engineering
- Viral vector production
Background:
- Continuous manufacturing is advancing biopharmaceutical production.
- Periodic counter-current chromatography (PCC) is a promising alternative to batch chromatography.
- Adeno-associated virus (AAV) therapies require high doses, necessitating efficient production methods.
Purpose of the Study:
- To implement and optimize a continuous AAV affinity capture process using a four-column PCC system.
- To improve the productivity and economics of AAV manufacturing.
Main Methods:
- Elution buffer screening for optimizing virus recovery.
- Generation and mechanistic modeling of breakthrough curves to characterize the PCC loading zone.
- Experimental runs to achieve a stable cyclic steady state.
Main Results:
- Stable cyclic steady state achieved in the four-column PCC process.
- Virus recoveries comparable to optimized batch processes (>82%).
- Nearly threefold improvement in productivity demonstrated.
Conclusions:
- The developed PCC affinity capture process enhances AAV manufacturing efficiency.
- This continuous process contributes to integrated continuous manufacturing concepts.
- Further improvements in process economics and manufacturing footprint are anticipated.
