Correlation of Histamine Receptors and Adrenergic Receptor in Broilers Appetite

The current study was conducted to investigate the interaction between the central adrenergic and histaminergic systems and the broiler chick's feed intake. In the first experiment, the intracerebroventricular (ICV) injection of solutions was conducted which included 10 nmol of prazosin (an α₁-receptor antagonist), 300 nmol of histamine, co-injection of prazosin and histamine. Experiments two to five were conducted similarly the same as the first experiment, in which chickens were ICV injected with 13 nmol of yohimbine (an α₂-receptor antagonist), 24 nmol of metoprolol (a β₁ adrenergic receptor antagonist), 5 nmol of ICI 118,551 (a β₂ adrenergic receptor antagonist), and 20 nmol of SR 59230R (a β₃ adrenergic receptor antagonist). The injected solutions in the sixth experiment included 300 nmol of noradrenaline, 250 nmol of α-FMH (an alpha fluoromethyl histidine), noradrenaline, and α-FMH. Seventh to ninth experiments were similar to the sixth experiment, except that the chickens were ICV injected with 300 nmol of chlorpheniramine (a histamine H₁ receptors antagonist), 82 nmol of famotidine (a histamine H₂ receptors antagonist), and 300 nmol of thioperamide (a histamine H₃ receptors antagonist), rather than α-FMH. Afterward, the cumulative food intake was measured 120 min after injection. Based on the obtained results, both histamine ICV injection and noradrenaline injection reduced food intake (P<0.05). Moreover, co-injection of histamine and ICI 118,551 (P<0.05), and co-injection of noradrenaline and Chlorpheniramine reduced food intake (P<0.05). In addition, noradrenaline and Thioperamide co-injection improved hypophagic effect of noradrenaline in neonatal chicken (P<0.05). These findings suggested the effect of interconnection between adrenergic and histaminergic systems, which may be mediated by H₁ and H₃ histaminergic and β₂ adrenergic receptors, on the regulation of food intake in the neonatal broiler chicken.