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Updated: Sep 3, 2025

Development of Heterogeneous Enantioselective Catalysts using Chiral Metal-Organic Frameworks MOFs
Published on: January 17, 2020
In situ modulation of enzyme activity via heterogeneous catalysis utilizing solid electroplated cofactors
N Apushkinskaya1, E V Zolotukhina2, E V Butyrskaya3
1Institute of Biochemistry, Saarland University, 66123, Saarbrücken, Campus B 2.2, Germany.
Abstract:
During product isolation the received bioreceptors often do not exhibit a sufficient biochemical activity due to multistep dissociation and loss of cofactors. However, for bioelectrochemical applications the presence of cofactors is necessary for a successful oxidative or reductive conversion of the substrates to the products. Herein, we show how the immobilization of the required electroplated cofactors in a design of amperometric electrodes can in situ assist the activity of apo-enzymes. Compared to conventional approaches used in enzyme engineering this tailored nanoengineering methodology is superior from economic point of view, labor and time costs, storage conditions, reduced amount of waste and can fill the gap in the development of tuned bioelectrocatalysts.
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