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Modulation of Multiple Gene Clusters' Expression by the PAS-LuxR Transcriptional Regulator PteF.
Cláudia M Vicente1,2,3, Tamara D Payero1,2, Antonio Rodríguez-García1,2
1Department of Molecular Biology, Area of Microbiology, Faculty of Biology, Universidad de León, 24071 León, Spain.
Antibiotics (Basel, Switzerland)
|July 27, 2022
Summary
The study reveals that PteF, a regulator in Streptomyces avermitilis, negatively modulates gene expression, impacting antifungal and antibiotic production. This challenges its prior classification as a transcriptional activator.
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- PAS-LuxR transcriptional regulators control polyene antifungal biosynthesis in bacteria.
- PteF from Streptomyces avermitilis regulates filipin biosynthesis and was previously thought to be an activator.
Purpose of the Study:
- To investigate the regulatory role of PteF in Streptomyces avermitilis.
- To understand the impact of PteF on gene expression, particularly secondary metabolite biosynthesis.
Main Methods:
- Transcriptomic analysis comparing a Streptomyces avermitilis ΔpteF mutant with its parental strain.
- Gene expression profiling at exponential and stationary growth phases.
- Validation of transcriptomic data using quantitative reverse-transcription polymerase chain reaction (qRT-PCR).
Main Results:
- Deletion of PteF altered the expression of 257 genes, predominantly at the exponential phase.
- Most affected genes were overexpressed in the mutant, indicating PteF acts as a negative modulator.
- PteF influences diverse metabolic pathways and regulates 10 out of 38 secondary metabolite gene clusters.
Conclusions:
- PteF functions as a negative transcriptional regulator, contrary to previous assumptions.
- PteF has a broad regulatory role in Streptomyces avermitilis, affecting antibiotic biosynthesis and other processes.
- Findings elucidate the complex regulatory network governing antibiotic production in Streptomyces.
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