Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Teriflunomide Attenuates Demyelination and Enhances Remyelination in Organotypic Brain Slice Cultures Through Modulation of Glial Cell Dynamics.

CNS neuroscience & therapeutics·2026
Same author

Resolving phenotyping discordance with SPACEMAP, an integrated machine learning framework.

bioRxiv : the preprint server for biology·2025
Same author

Effect of Reoxygenation on Radioresistance of Chronically Hypoxic A549 Non-Small Cell Lung Cancer (NSCLC) Cells Following X-Ray and Carbon Ion Exposure.

International journal of molecular sciences·2025
Same author

DNA Damage and Inflammatory Response of p53 Null H358 Non-Small Cell Lung Cancer Cells to X-Ray Exposure Under Chronic Hypoxia.

International journal of molecular sciences·2024
Same author

Omics Studies of Specialized Cells and Stem Cells under Microgravity Conditions.

International journal of molecular sciences·2024
Same author

Space radiation measurements during the Artemis I lunar mission.

Nature·2024

Related Experiment Video

Updated: Sep 3, 2025

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
08:07

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

73.4K

Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies.

Sebastian Feles1,2, Christian Overath3, Sina Reichardt4

  • 1Radiation Biology, Institute of Aerospace Medicine, German Aerospace Center (DLR), 51147 Cologne, Germany.

Methods and Protocols
|July 27, 2022
PubMed
Summary

This study provides a comprehensive guide for culturing SH-SY5Y neuroblastoma cells, a key model for neurodevelopmental disorders. Optimized protocols ensure stable cell growth and differentiation for reproducible neuroscience research.

Keywords:
SH-SY5Ycell culturecharacterizationcultivation techniquesin vitro methodsmorphologyneuroblastomaneuroscienceproliferation

More Related Videos

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.9K
High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

17.6K

Related Experiment Videos

Last Updated: Sep 3, 2025

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
08:07

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line

Published on: February 17, 2016

73.4K
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
13:47

TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis

Published on: January 29, 2015

10.9K
High Content Screening in Neurodegenerative Diseases
13:32

High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

17.6K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biotechnology

Background:

  • SH-SY5Y cells are a widely used in vitro model for studying neurodevelopmental disorders like Parkinson's disease.
  • Existing cultivation methods for SH-SY5Y cells are varied and sometimes contradictory.
  • This cell line offers advantages over primary neuronal cultures due to its differentiation capacity.

Purpose of the Study:

  • To provide a comprehensive guide for the in vitro cultivation of undifferentiated SH-SY5Y cells.
  • To detail cell morphology, differentiation of subtypes, and best practices for cell culture and cryopreservation.
  • To establish optimized protocols for stable, long-term cultivation and reproducible differentiation.

Main Methods:

  • Characterization of SH-SY5Y cell growth, including proliferation, confluency, and optimal seeding densities.
  • Comparative analysis of different culture media and assessment of cell viability.
  • Long-term cultivation over 60 days using an optimized protocol to evaluate cumulative population doubling (CPD).

Main Results:

  • Detailed descriptions of SH-SY5Y cell morphology and differentiation characteristics.
  • Identification of optimal conditions for cell culture, including media and seeding numbers.
  • Demonstration that CPD remains constant over extended cultivation periods (60 days), indicating stable proliferation across passages.
  • Successful long-term cryopreservation protocols are provided.

Conclusions:

  • This study offers a standardized and optimized protocol for SH-SY5Y cell cultivation, enhancing reproducibility in neuroscience research.
  • The findings support the use of SH-SY5Y cells for consistent and reliable in vitro studies of neurodevelopmental disorders.
  • The provided guidance ensures stable cell line maintenance for recurrent differentiation and subsequent analyses.