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Updated: Sep 3, 2025
A Fast Air-dry Dropping Chromosome Preparation Method Suitable for FISH in Plants
Published on: December 16, 2015
Developing Oligo Probes for Chromosomes Identification in Hemp (Cannabis sativa L.)
Dmitry V Romanov1, Gennady I Karlov1, Mikhail G Divashuk1
1All-Russia Research Institute of Agricultural Biotechnology, 127550 Moscow, Russia.
Abstract:
Hemp (Cannabis sativa L., 2n = 20) is a valuable crop that is successfully used as a food, technical and medicinal crop. It is a dioecious plant with an XX\XY sex determination system. Some chromosomes of C. sativa have almost the same lengths and centromeric indexes. Cytogenetic markers help to distinguish similar plant chromosomes, including sex chromosomes, which is important for the breeding process. Two repeats (CS-1 and CS-237) were used to develop labeled oligo-probes for rapid and low-cost oligo-FISH. These oligos can be recommended for use as cytological markers to distinguish sex chromosomes (X and Y) and somatic chromosome pairs 3, 6, and 8 by rapid oligo-FISH in a short time.
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