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Published on: February 6, 2015
Effect of Chloroquine on Doxorubicin-induced Apoptosis in A549 Cells
Keisuke Sato1, Natsuki Ota1, Shoya Endo1
1Department of Pharmacy, Hokkaido University of Science, Sapporo, Japan.
Background/Aim:
We investigated the effects of chloroquine, an autophagy inhibitor, on doxorubicin-induced apoptosis in A549 cells.
Materials And Methods:
A549 cells were treated with doxorubicin, chloroquine, or both. Then, cytotoxicity was measured. The expression levels of caspases and mitogen-activated protein kinases were also quantified. In addition, the levels of doxorubicin-derived reactive oxygen species were measured.
Results:
Chloroquine enhanced doxorubicin-induced apoptosis and oxidative stress and suppressed the doxorubicin-induced extracellular-signal-regulated kinase activation.
Conclusion:
Chloroquine enhances doxorubicin-induced and oxidative stress-mediated apoptosis. This mechanism may involve the dephosphorylation of extracellular-signal-regulated kinases.
