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Overexpression of splicing factor poly(rC)-binding protein 1 elicits cycle arrest, apoptosis induction, and p73

Yuhong Chen1,2,3,4, Zhihui Dou1,2,3,4, Xiaohua Chen1,2,3,4

  • 1Bio-Medical Research Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.

Journal of Cancer Research and Clinical Oncology
|July 27, 2022
PubMed
Abstract

Insights

Splicing factor PCBP1 suppresses cervical cancer growth by inducing apoptosis and cell cycle arrest. Targeting PCBP1 may offer a new therapeutic strategy for cervical cancer.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Biology

Background:

  • Splicing factor poly(rC)-binding protein 1 (PCBP1) is a tumor suppressor implicated in cancer progression.
  • The specific role of PCBP1 in cancer cell apoptosis and its underlying mechanisms are not fully understood.

Purpose of the Study:

  • To investigate the function of PCBP1 in apoptosis of human cervical cancer cells.
  • To elucidate the molecular mechanisms by which PCBP1 influences cancer cell fate.

Main Methods:

  • Overexpression of PCBP1 in human cervical cancer cells (HeLa).
  • Assessment of cell proliferation, cell cycle progression, and apoptosis using cytological assays.

Main Results:

  • PCBP1 overexpression significantly inhibited HeLa cell proliferation in a time-dependent manner.
  • PCBP1 induced G2/M phase arrest and increased apoptosis.
  • PCBP1 promoted the production of long p73 isoforms, leading to an altered Bax/Bcl-2 ratio, cytochrome c release, and caspase-3 activation.

Conclusions:

  • PCBP1 plays a critical role in regulating p73 splicing, cell cycle arrest, and apoptosis in cervical cancer cells.
  • PCBP1 represents a potential therapeutic target for cervical cancer treatment.
Keywords:
ApoptosisCancerPCBP1SplicingTherapyp73

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