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Effect of pH on Diclofenac-Lysozyme Interaction: Structural and Functional Aspect
Mohd Basheeruddin1, Sheeza Khan1, Neesar Ahmed1
1School of Life Sciences, B. S. Abdur Rahman Crescent Institute of Science and Technology, Chennai, India.
Frontiers in Molecular Biosciences
|July 28, 2022
Summary
Diclofenac (DCF) destabilizes lysozyme structure and function. This nonsteroidal anti-inflammatory drug
Area of Science:
- Biochemistry
- Pharmacology
Background:
- Diclofenac (DCF) is a nonsteroidal anti-inflammatory drug (NSAID) with known side effects.
- Long-term NSAID use can lead to cardiovascular and renal complications.
Purpose of the Study:
- To investigate the impact of diclofenac on the structural integrity, stability, and function of lysozyme.
- To analyze diclofenac's effects across various pH conditions.
Main Methods:
- Heat-induced denaturation of lysozyme was monitored.
- Thermodynamic parameters (Tm, ΔHm, ΔCp, ΔGDo) were determined in the presence and absence of varying diclofenac concentrations at different pH values.
Main Results:
- Diclofenac destabilized lysozyme, indicated by changes in Tm and ΔGDo across all tested pH values.
- Protein destabilization was less pronounced at acidic pH (2.0) compared to physiological pH (7.0).
- Structural changes were less significant at pH 2.0 than pH 7.0, with quenching observed at both pH values.
Conclusions:
- Diclofenac negatively affects lysozyme stability and structure.
- The destabilizing effect of diclofenac on lysozyme is entropically driven.
- Understanding these interactions is crucial for evaluating NSAID safety and potential therapeutic applications.
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