Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Gene Regulation During Sporulation

Gene Regulation During Sporulation

Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Cell Signaling in Plants

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...

Riboswitches

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

Regulation of Nuclear Protein Sorting

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...

Global Regulatory Systems

Global Regulatory Systems

Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

OsvWA36 transcriptionally regulates cell wall remodeling to control grain length in rice.

BMC plant biology·2026

Same author

Liquid-liquid phase separation of OsvWA36 fine-tunes brassinosteroid signaling and leaf angle in rice.

Plant physiology and biochemistry : PPB·2026

Same author

Identifying Heat Adaptability QTLs and Candidate Genes for Grain Appearance Quality at the Flowering Stage in Rice.

Rice (New York, N.Y.)·2025

Same author

Mechanistic Insights into Lignin's Inhibition of Gluten Digestibility: Evidence from In Vitro Static Digestion.

Journal of agricultural and food chemistry·2024

Same author

WD40 protein OsTTG1 promotes anthocyanin accumulation and CBF transcription factor-dependent pathways for rice cold tolerance.

Plant physiology·2024

Same author

TMT-based quantitative proteomics analysis of defense responses induced by the Bph3 gene following brown planthopper infection in rice.

BMC plant biology·2024

Search research articles

Related Experiment Video

Updated: Sep 3, 2025

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

OsSPL88 Encodes a Cullin Protein that Regulates Rice Growth and Development.

Zhengai Chen¹, Wenjing Yin¹, Xuan Li¹

¹College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua, China.

Frontiers in Genetics

|July 28, 2022

Summary

This summary is machine-generated.

Two rice mutants, spl88-1 and spl88-2, exhibit spontaneous lesions due to reactive oxygen species (ROS) accumulation. The SPL88 gene, encoding a Cullin protein, regulates plant defense and development.

Keywords:

SPL88 cullin protein growth and development lesion mimics rice

More Related Videos

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Related Experiment Videos

Last Updated: Sep 3, 2025

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

A Strategy to Validate the Role of Callose-mediated Plasmodesmal Gating in the Tropic Response

Published on: April 17, 2016

Area of Science:

Plant Science
Genetics
Molecular Biology

Background:

Plant lesion mimics are necrotic spots appearing without external triggers.
Understanding their genetic basis is crucial for crop improvement.

Purpose of the Study:

To identify and characterize genes responsible for spontaneous lesion mimicry in rice.
To investigate the role of the identified gene in plant defense and development.

Main Methods:

Ethyl methanesulfonate mutagenesis and screening of rice mutants.
Physiological and biochemical analyses for reactive oxygen species (ROS) detection.
Map-based cloning to isolate the causative gene.
Gene expression and protein localization studies.

Main Results:

Two rice mutants, spl88-1 and spl88-2, were identified with spontaneous lesions.
Mutants showed increased ROS accumulation, spontaneous cell death, and enhanced resistance to bacterial blight.
Map-based cloning identified the SPL88 gene, encoding a Cullin protein, with premature stop codons in mutants.
SPL88 is expressed in various rice tissues and its protein localizes to the cytoplasm and nucleus.

Conclusions:

The SPL88 gene regulates rice growth and development.
SPL88 plays a role in plant defense mechanisms by influencing defense-related gene expression.