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Application Progress of Massively Parallel Sequencing Technology in STR Genetic Marker Detection.
Rui-Yang Tao1, Xin-Yu Dong1, An-Qi Chen1
1Shanghai Key Laboratory of Forensic Medicine, Key Laboratory of Forensic Science, Ministry of Justice, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
Fa Yi Xue Za Zhi
|July 28, 2022
Summary
Massively parallel sequencing (MPS) offers advanced forensic genetics capabilities for short tandem repeat (STR) analysis, providing higher resolution and efficiency. Challenges include cost, standardization, and integrating new data with existing databases.
Area of Science:
- Forensic Genetics
- Molecular Biology
- Biotechnology
Background:
- Forensic genetics laboratories are increasingly adopting Massively Parallel Sequencing (MPS), also known as Next-Generation Sequencing (NGS).
- Short Tandem Repeats (STRs) are crucial forensic genetic markers, traditionally analyzed via capillary electrophoresis (CE).
- MPS technology enables simultaneous detection of numerous genetic markers and high-throughput sample processing.
Purpose of the Study:
- To review the current applications of MPS technology in detecting STR genetic markers for forensic purposes.
- To identify and discuss the primary challenges hindering widespread MPS adoption in forensic genetics.
- To explore the future prospects of MPS technology in forensic genetic applications.
Main Methods:
- Review of existing literature and case studies on MPS application in forensic genetics.
- Comparative analysis of MPS technology versus traditional capillary electrophoresis (CE) for STR analysis.
- Identification of technical and logistical challenges associated with MPS implementation.
Main Results:
- MPS offers superior resolution and efficiency for STR analysis compared to CE.
- MPS allows for simultaneous analysis of a large number of genetic markers and samples.
- Key challenges include high costs, lack of standardized protocols, and data integration with existing CE-STR databases.
Conclusions:
- MPS technology presents significant advantages for forensic STR analysis, including enhanced resolution and throughput.
- Addressing challenges related to cost, standardization, and database integration is crucial for broader adoption.
- The future of forensic genetics is likely to involve the integration and advancement of MPS technologies.
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