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Updated: Sep 3, 2025

Gene Editing of Primary Rhesus Macaque B Cells
Published on: February 10, 2023
Reprogramming of Human B Cells from Secreting IgG to IgM by Genome Editing
Guigao Lin1,2,3, Kuo Zhang1,2,3, Yanxi Han1,3
1National Center for Clinical Laboratories, Institute of Geriatric Medicine, Chinese Academy of Medical Sciences, Beijing Hospital/National Center of Gerontology, PR China; Beijing Hospital, Beijing, PR China.
Abstract:
B lymphocytes are activated and regulated by their interactions with T cells, a process that results in one-way class switching of immunoglobulins (ig) from IgM to IgG, IgE, or IgA. In this study, we show the application of clustered regularly interspaced short palindromic repeat-Cas9-induced nonhomologous end joining in B cells to achieve reverse-directional Ig class switching. By electroporating Cas9 and guide RNA and a Cμ encoding donor into cells, we engineered IgG-secreting human B cell lines to switch to express IgM antibody. This approach offers a new potential path for the production of IgM antibodies.
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