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Green vortex-assisted emulsification microextraction using a ternary deep eutectic solvent for extraction of
Hassan Sereshti1, Mohammad Zarei-Hosseinabadi1, Sara Soltani1
1School of Chemistry, College of Science, University of Tehran, Tehran, Iran.
Food Chemistry
|July 28, 2022
Summary
A new deep eutectic solvent (DES) method efficiently extracts tetracyclines from infant formula. This green chemistry approach offers high recovery rates for accurate analysis.
Area of Science:
- Analytical Chemistry
- Green Chemistry
Background:
- Tetracyclines are antibiotics used in animal husbandry, raising concerns about their presence in infant formula.
- Accurate and sensitive analytical methods are crucial for monitoring tetracycline residues in food products.
Purpose of the Study:
- To develop a novel, green, and efficient method for extracting tetracyclines from infant formula.
- To synthesize and evaluate new hydrophobic deep eutectic solvents (DESs) for this extraction process.
Main Methods:
- Vortex-assisted emulsification liquid-liquid microextraction using a novel ternary DES.
- High-performance liquid chromatography (HPLC) for tetracycline analysis.
- Characterization of the DES using FTIR, NMR, and octanol-water partition coefficient.
Main Results:
- A thymol:ethylene glycol:benzyl alcohol (2:2:1) DES showed the highest extraction efficiency.
- Optimized method achieved limits of quantification of 3-9.32 μg kg⁻¹.
- Recoveries ranged from 68.9-102.0% with relative standard deviations < 9% in infant formula samples.
Conclusions:
- The developed method is effective and green for tetracycline analysis in infant formula.
- This represents the first application of a novel ternary DES for tetracycline determination in this matrix.
