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Updated: Sep 3, 2025

Identification of Olfactory Volatiles using Gas Chromatography-Multi-unit Recordings GCMR in the Insect Antennal Lobe
Published on: February 24, 2013
Identification of Bioactive Volatile Organic Compounds Using Combined Gas Chromatography and Single Sensillum
Rickard Ignell1, Majid Ghaninia2,3, Sharon Rose Hill4
1Disease Vector Group, Unit of Chemical Ecology, Department of Plant Protection Biology, Swedish University of Agricultural Sciences, 23422 Alnarp, Sweden rickard.ignell@slu.se.
Abstract:
Most arthropods rely heavily on their sense of smell (i.e., olfaction) to locate and discriminate among mates, food, and egg laying sites. The odors emanating from these resources are composed of blends of volatile compounds that are detected by olfactory sensory neurons (OSNs) that are housed in hair-like structures, called sensilla, on the olfactory organs of arthropods. By inserting an electrode into a single sensillum and recording the activity of the OSNs while stimulating with volatile compounds eluting from a gas chromatograph, combined gas chromatography and single sensillum recording (GC-SSR) provides a high-resolution tool to identify bioactive compounds and to functionally characterize the peripheral olfactory system of arthropods.
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