Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Glycol methacrylate as an embedding medium for bone.

M Hott, P J Marie

    Stain Technology
    |January 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Endothelin-1 Augments Therapeutic Potency of Human Mesenchymal Stem Cells via CDH2 and VEGF Signaling.

    Molecular therapy. Methods & clinical development·2019
    Same author

    Zinc chelation: a metallothionein 2A's mechanism of action involved in osteosarcoma cell death and chemotherapy resistance.

    Cell death & disease·2013
    Same author

    E3 ubiquitin ligase-mediated regulation of bone formation and tumorigenesis.

    Cell death & disease·2013
    Same author

    Cell and gene therapy for bone repair.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2011
    Same author

    [CT scan, MR imaging and anatomopathologic correlation in the glottic carcinoma T1-T2].

    Revue de laryngologie - otologie - rhinologie·2010
    Same author

    How strontium ranelate, via opposite effects on bone resorption and formation, prevents osteoporosis.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2010
    Same journal

    Further observations on ethyl alcohol substitutes.

    Stain technology·2010
    Same journal

    Sterile milk for background in capsule staining.

    Stain technology·2010
    Same journal

    The use of clarite in preparing whole microscopic mounts.

    Stain technology·2010
    Same journal

    A simplified method of preparation of di-ammine-silver hydroxide for reticulum impregnation; comments on the nature of the so-called sensitization before impregnation.

    Stain technology·2010
    Same journal

    A simplified stain for hemoglobin in tissues or smears using patent blue.

    Stain technology·2010
    Same journal

    Improved fixation in vitally stained methylene blue preparations.

    Stain technology·2010
    See all related articles

    A new, cost-effective glycol methacrylate (GMA) embedding method for undecalcified bone tissue has been developed. This reliable procedure preserves tissue and enzymatic activity, enabling detailed histomorphometry and marker visualization.

    Area of Science:

    • Biomaterials Science
    • Histology
    • Biochemistry

    Background:

    • Undecalcified bone tissue embedding is crucial for histomorphometry and metabolic studies.
    • Existing embedding methods can be expensive or compromise tissue integrity and enzymatic activity.

    Purpose of the Study:

    • To develop a simple, reliable, and cost-effective noncommercial glycol methacrylate (GMA) embedding procedure for undecalcified trabecular bone.
    • To ensure the method preserves tissue structure, allows for enzymatic activity demonstration, and facilitates histomorphometric analysis.

    Main Methods:

    • Developed a novel embedding mixture using specific proportions of glycol methacrylate (GMA) monomer, copolymer, cross-linker, catalyst, initiator, and heat moderator.
    • Optimized polymerization at -4°C to preserve enzymatic activity.

    Related Experiment Videos

  • Evaluated sectioning ease, specimen swelling, and water surface spreading characteristics.

    • Main Results:

    • The developed GMA embedding procedure is simple, reliable, and cost-effective compared to commercial kits.
    • Specimens are easily sectioned with classical microtomes, do not swell, and spread evenly on water.
    • The low-temperature polymerization (-4°C) preserves enzymatic activities (acid/alkaline phosphatase, carbonic anhydrase) and allows for excellent bone tissue preservation.
    • The method supports histomorphometry, fluorescent labeling, and radioactive marker visualization for bone metabolism studies.

    Conclusions:

    • The noncommercial GMA embedding method offers a superior alternative for undecalcified bone tissue preparation.
    • This technique provides excellent tissue preservation, retains enzymatic activity, and is suitable for advanced analyses in bone research.
    • Glycol methacrylate (GMA) embedding is recommended over methyl methacrylate for routine bone tissue preparation.