Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptojanin1 regulates synaptic dopamine release and axonal integrity via retromer-dependent endosomal sorting.

Research square·2026
Same author

Recombinant dimeric PICK1 peptide inhibitors for long-term relief of chronic pain by AAV therapeutics.

Cell reports. Medicine·2026
Same author

Ultrastructure of dopaminergic varicosities revealed by cryo-correlative light and electron microscopy.

The Journal of cell biology·2026
Same author

Visual attention is linked to temporally structured noradrenaline release in the medial prefrontal cortex.

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology·2026
Same author

Pearling drives mitochondrial DNA nucleoid distribution.

Science (New York, N.Y.)·2026
Same author

Brain-wide mapping reveals temporal and sexually dimorphic opioid actions.

Communications biology·2026

Related Experiment Video

Updated: Jun 22, 2026

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
12:58

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

Published on: October 24, 2010

18.3K

A density-based enrichment measure for assessing colocalization in single-molecule localization microscopy data.

Aske L Ejdrup1, Matthew D Lycas2, Niels Lorenzen2

  • 1Department of Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. ejdrup@sund.ku.dk.

Nature Communications
|July 28, 2022
PubMed
Summary

This study introduces a new method for analyzing dual-color single-molecule localization microscopy (SMLM) data to better understand molecular colocalization. The tool provides a richer, context-aware analysis beyond single values, visualizing molecular enrichment directly in images.

More Related Videos

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.8K

Related Experiment Videos

Last Updated: Jun 22, 2026

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging
12:58

Visualisation and Quantification of Intracellular Interactions of Neisseria meningitidis and Human α-actinin by Confocal Imaging

Published on: October 24, 2010

18.3K
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.0K
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
07:48

Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis

Published on: July 3, 2015

8.8K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Dual-color single-molecule localization microscopy (SMLM) enables detailed molecular colocalization studies.
  • Current SMLM analysis tools often oversimplify colocalization into single values, limiting data interpretation.

Purpose of the Study:

  • To develop a novel computational tool for precise 2D and 3D colocalization analysis of SMLM data.
  • To provide a more informative and context-relevant measure of molecular co-enrichment.

Main Methods:

  • Developed a function to quantify relative molecular species enrichment within a reference species' density distribution.
  • Implemented a visualization technique to display colocalization directly within SMLM images.
  • Validated the method using simulated data and cultured neurons.

Main Results:

  • The new method offers a density-contextualized understanding of colocalization.
  • Direct image visualization facilitates intuitive interpretation of molecular enrichment.
  • Demonstrated functional equivalence and potential advantages over existing colocalization measures.

Conclusions:

  • The developed tool enhances the analytical capabilities for dual-color SMLM data.
  • Provides a versatile and user-friendly approach for quantitative colocalization analysis.
  • Facilitates deeper insights into molecular interactions within cellular environments.